Vancomycin Resistant Enterococci (VRE) Agar Base

Vancomycin Resistant Enterococci (VRE) Agar Base is recommended for the selective isolation of Vancomycin Resistant Enterococci. Enterococci are part of the normal microbial flora of mammalian intestines and the presence of enterococci is an indication of faecal contamination1. Vancomycin-resistant Enterococcus (VRE) is the name given to a group of bacterial species of the genus Enterococcus that are resistant to the antibiotic vancomycin. Vancomycin, is the treatment for the penicillin- and aminoglycoside-resistant enterococcal infections.

This media is formulated as per the recommendations of Centre for Disease Control and Prevention (CDC) for the selective isolation of vancomycin resistant enterococci2. Enterococci species hydrolyze esculin to glucose and esculetin. The resultant esculetin reacts with ferric ions to form a dark brown or black complex visualized as a zone of black precipitate around the colonies. Sodium azide inhibits most of the accompanying microflora. Vancomycin helps in the selective isolation of vancomycin resistant enterococci from other enterocci and Meropenem helps to suppress the contaminating flora especially gram-negative bacteria. Early detection of VRE is important for infection control and prevention measures, epidemiological infectious disease follow-up, and also prevention of vancomycin resistant Staphylococcus aureus emergence3.

Product NameCatalog #Quantity
VRE Agar w/Vancomycin mono plate, 90 x 15 mm CV9580P 10/pk
BSA Agar Slant, w/Vancomycin CV9580S 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Enterococcus faecalis (VRE) ATCC® 51299 Good growth, light blue colonies
Enterococcus faecalis (VRE) ATCC® 12201 Good growth, light blue colonies
Enterococcus faecium (VRE) ATCC® 12202 Good growth, indigo/purple colonies
Enterococcus faecalis ATCC® 35667 Inhibited
Enterococcus faecium ATCC® 19433 Inhibited
Enterococcus gallinarum ATCC® 700425 Inhibited

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.0 +/- 0.2 at 25°C)
Tryptone 20.0
Yeast Extract 5.0
Sodium Chloride 5.0
Yeast Extract 5.0
Oxgall 10.0
Esculin 1.0
Ferric Ammonium Citrate 0.5
Sodium Azide 0.25
Sodium Citrate 1.0
Oxgall 10.0
Vancomycin 0.008
Agar 10.0
3 total citations

  1. Mara D., Horan NJ : The Handbook of water, wastewater and microbiology , Amsterdam, The Netherlands , Academic Press ; 2003
  2. CDC Preventing the spread of vancomycin resistance: a report from the Hospital Infection Control Practices Advisory Committee(1994). Fed Regist. May17
  3. Mascini EM, Bonten MJ : Vancomycin- resistant enterococci : consequences for therapy and infection control . Clin Microbiol Infect.2005,11 (Suppl.4) :43-56
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