Tryptic Soy Agar
Tryptic Soy Agar is a partially completed general purpose medium which is recommended for use as a general growth medium for the isolation and cultivation of microorganisms, and also for use in the cultivation, storage, maintenance and transportation of pure cultures of microorganisms. The formulation of Tryptic Soy Agar contains digests of soybean meal and casein , and is prepared according to the United States Pharmacopeia (USP) standard formula for Soybean-Casein Digest Agar Medium1, Compendium of Methods for the Microbiological Examination of Foods and in Standard Methods for the Examination of Water and Wastewater2,3.
The soy and casein peptones supply organic nitrogen in the form of amino acids and polypeptides, making the medium highly nutritious. This all-purpose growth medium serves as the base for other media formulations and may be supplemented with blood to support the growth of fastidious microorganisms, or with antimicrobials to provide selective medium for specific organisms in a mixed flora testing.
|Product Name||Catalog #||Quantity|
|Tryptic Soy Agar mono plate, 90 x 15 mm||CT9530P||10/pk|
|Tryptic Soy D-Slant||CT9530S||10/pk|
|Tryptic Soy Broth||CT9530B||10/pk|
The following organisms are routinely used for testing for this medium.
|Staphylococcus aureus ATCC® 25923||Growth|
|Escherichia coli ATCC® 25922||Growth|
|Candida albicans ATCC® 10231||Growth|
|Bacillus subtilis ATCC® 6633||Growth|
|Aspergillus brasiliensis ATCC® 16404 formerly A. niger||Growth|
User Quality Control
Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).
Ingredients g/L(Final pH 7.3 +/- 0.2 at 25°C)
|Pancreatic Digest of Casein||15.0|
|Peptic Digest of Soybean Meal||5.0|
3 total citations
- The Official Compendia of Standards. 2008. USP27-NF22. United States Pharmacopeial Convention, Rockville, MD.
- American Public Health Association. 2005. Standard Methods for the Examination of Water and Wastewater, 21st ed. APHA, Washington, D.C.
- APHA Technical Committee on Microbiological Methods for Foods. 2001. Compendium of Methods for the Microbiological Examination of Foods, 4th ed. APHA, Washington, D.C.