Tryptic Soy Agar

Tryptic Soy Agar is a partially completed general purpose medium which is recommended for use as a general growth medium for the isolation and cultivation of microorganisms, and also for use in the cultivation, storage, maintenance and transportation of pure cultures of microorganisms. The formulation of Tryptic Soy Agar contains digests of soybean meal and casein , and is prepared according to the United States Pharmacopeia (USP) standard formula for Soybean-Casein Digest Agar Medium1, Compendium of Methods for the Microbiological Examination of Foods and in Standard Methods for the Examination of Water and Wastewater2,3.

The soy and casein peptones supply organic nitrogen in the form of amino acids and polypeptides, making the medium highly nutritious. This all-purpose growth medium serves as the base for other media formulations and may be supplemented with blood to support the growth of fastidious microorganisms, or with antimicrobials to provide selective medium for specific organisms in a mixed flora testing.


Product NameCatalog #Quantity
Tryptic Soy Agar mono plate, 90 x 15 mm CT9530P 10/pk
Tryptic Soy D-Slant CT9530S 10/pk
Tryptic Soy Broth CT9530B 10/pk


Quality Control



The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Staphylococcus aureus ATCC® 25923 Growth
Escherichia coli ATCC® 25922 Growth
Candida albicans ATCC® 10231 Growth
Bacillus subtilis ATCC® 6633 Growth
Aspergillus brasiliensis ATCC® 16404 formerly A. niger Growth


User Quality Control



Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.3 +/- 0.2 at 25°C)
Pancreatic Digest of Casein 15.0
Peptic Digest of Soybean Meal 5.0
Sodium Chloride 5.0
Agar 15.0
3 total citations

  1. The Official Compendia of Standards. 2008. USP27-NF22. United States Pharmacopeial Convention, Rockville, MD.
  2. American Public Health Association. 2005. Standard Methods for the Examination of Water and Wastewater, 21st ed. APHA, Washington, D.C.
  3. APHA Technical Committee on Microbiological Methods for Foods. 2001. Compendium of Methods for the Microbiological Examination of Foods, 4th ed. APHA, Washington, D.C.
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