Thayer Martin Agar

Thayer Martin Agar is a selective and enriched medium for the isolation and cultivation of Neisseria sp. from mixed flora with suppression of most other gram-negative diplococci, gram-negative bacilli, gram-positive organisms, and yeast. Hemoglobin, Bio-X and dextrose are the nutrients agents to permit luxuriant growth of fastidious microorganisms. Antibiotics inhibit normal flora growth as yeasts, gram positive and gram negative bacteria. Mixture of vancomycin and lincomycin prevents inhibition of Neisseria gonorrhoeae by high concentration of vancomycin1. Thayer and Martin reported an improvement of the Chocolate Agar formulation that contained antimicrobics vancomycin, colistin and nystatin1,2. Martin and Lester later added antibiotic trimethoprim to make the medium further selective and shown to be of value in the suppression of Proteus spp3. Trimethoprim lactate refrains Proteus swarming.The resulting medium is called Modified Thayer Martin.


Product NameCatalog #Quantity
Thayer Martin Agar mono plate, 90 x 15 mm CT9510P 10/pk
Modified Thayer Martin Agar mono plate, 90 x 15 mm CT9515P 10/pk
Thayer Martin Slant CT9510S 10/pk
Thayer Martin Broth CT9510B 10/pk


Quality Control



The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Neisseria gonorrhoeae ATCC® 43069 Growth
Neisseria meningitides ATCC® 13090 Growth
Staphylococcus epidermidis ATCC® 12228 Partial to complete inhibition
Proteus mirabilis ATCC® 43071 Partial to complete inhibition; no swarming
Escherichia coli ATCC® 25922 Partial to complete inhibition
Candida albicans ATCC® 60193 Partial to complete inhibition
Neisseria sicca ATCC® 9913 Inhibited
Thayer Martin, Modified, with Vancomycin  
Neisseria gonorrhoeae ATCC® 43069 Growth
Neisseria meningitides ATCC® 13090 Growth
Staphylococcus epidermidis ATCC® 12228 Partial to complete inhibition
Escherichia coli ATCC® 25922 Partial to complete inhibition
Candida albicans ATCC® 60193 Partial to complete inhibition
Neisseria sicca ATCC® 9913 Inhibited


User Quality Control



Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.2 +/- 0.2 at 25° C)
Proteose Peptone No. 3 15.0
Hemoglobin, Bovine 10.0
Sodium Chloride 5.0
Dipotassium Phosphate 4.0
Monopotassium Phosphate 1.0
Corn Starch 1.0
Colistin 0.0075
Trimethoprim Lactate* 0.005
Vancomycin 0.003
Nystatin (Units) 1,250.0
KoEnzyme Enrichments 10.0 ml
Agar 12.0
3 total citations

  1. Thayer JD, Martin JE (1964). "A selective medium for the cultivation of N. gonorrhoeae and N. meningitidis".Public Health Rep 79: 49–57
  2. Thayer JD, Martin JE (June 1966). "Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis". Public Health Rep, 81 (6): 559–62
  3. Martin J. E. Jr. and Lester A., 1971, HSMHA Health. Service Rep., 86(1): 30
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