Nutrient Medium

Nutrient media are basic media used for the cultivation of less fastidious microorganisms, but can be used for cultivating fastidious organisms by enriching with different biological fluids such as horse or sheep blood, serum, egg yolk etc.1. This relatively simple formula has been retained and is still widely used in the microbiological examination, useful in routine cultivation of microorganisms2,3. This is the most popular medium for demonstration and teaching purposes where a more prolonged survival of cultures at ambient temperature is often required without risk of overgrowth that can occur with more nutritious substrate.

Product NameCatalog #Quantity
Nutrient Agar mono plate, 90 x 15 mm CN9410P 10/pk
Nutrient Agar mono plate, 90 x 15 mm Glutamine & Sodium bicarbonate CN9411P 10/pk
Nutrient Slant CN9410S 10/pk
Nutrient Broth CN9410B 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Escherichia coli (VRE) ATCC® 25922 Growth
Staphylococcus aureus (VRE) ATCC® 25923 Growth
Pseudomonas aeruginosa (VRE) ATCC® 27853 Growth

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.4 +/- 0.2 at 25°C)
Acid Hydrolysate of Casein 5.0
Sodium Chloride 5.0
Beef extract 1.5
Yeast extract 1.5
Agar (where applicable) 15.0
3 total citations

  1. Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, Academic Press, London.
  2. Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C.
  3. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.
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