Mueller Hinton Agar
Mueller Hinton Media is recommended for use in the cultivation of a wide variety of microorganisms and for disk diffusion sensitivity testing of non-fastidious organisms. Mueller Hinton Broth is recommended for preparing suspensions of microorganisms for disk diffusion sensitivity testing. Mueller and Hinton developed this medium to be a protein free medium for isolating pathogenic strains of Neisseria1 and it was found that Mueller Hinton Agar was useful in identifying sulfonimide-resistant and responsive strains of gonococci2. Also this media has been used in standardized antimicrobial disk susceptibility testing, as described by Bauer, Kirby, et al3.
Mueller Hinton Media contains beef infusion, casamino acids, and starch. Starch acts as a colloid that protects against toxic material in the medium and agar is added when a solidifying agent is needed. The Kirby-Bauer antimicrobial disk diffusion procedure is used with Mueller Hinton Agar plates. An antimicrobial impregnated disk is placed on an agar surface, resulting in diffusion of the antimicrobial into the surrounding medium. Effectiveness of the antimicrobial can be shown by measuring the zone of inhibition for a pure culture of an organism4,5. Mueller Hinton Broth is the same formulation, without the added agar. It is used for the cultivation of microorganisms, and for making dilutions of organisms to be used in the Kirby-Bauer disk diffusion studies.
|Product Name||Catalog #||Quantity|
|Mueller Hinton Agar mono plate, 90 x 15 mm||CM9380P||10/pk|
|Mueller Hinton Slant||CM9380S||10/pk|
|Mueller Hinton Broth||CM9380B||10/pk|
The following organisms are routinely used for testing for this medium.
|Escherichia coli (VRE) ATCC® 25922||Growth|
|Escherichia coli (VRE) ATCC® 35218||Growth|
|Staphylococcus aureus (VRE) ATCC® 25923||Growth|
|Pseudomonas aeruginosa (VRE) ATCC® 27853||Growth|
|Enterococcus faecalis (VRE) ATCC® 29212||Growth|
User Quality Control
Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).
Ingredients g/L(Final pH 7.3 +/- 0.1 at 25°C)
|Acid Hydrolysate of Casein||17.5|
|Agar (where applicable)||17.0|
5 total citations
- Mueller, J.H. and J. Hinton. 1941. A protein-free medium for primary isolation of the Gonococcus and Meningococcus. Proc. Soc. Exp. Diol. and Med.; 48:330-333.
- Ryan, K.J., et al. 1970. Disk sensitivity testing. Hosp. Prac.; 5:91-100.
- Bauer, A.W., W.M.M. Kirby, et al. 1966. Am. J. Clin. Pathol.; 45:493-496.
- Performance Standards for Antimicrobial Disk Susceptibility Tests. M2-A. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.
- Standard Disk Susceptibility Test. The Federal Register, September 30, 1972; 37(191):20527-20529