Mueller Hinton Agar

Mueller Hinton Media is recommended for use in the cultivation of a wide variety of microorganisms and for disk diffusion sensitivity testing of non-fastidious organisms. Mueller Hinton Broth is recommended for preparing suspensions of microorganisms for disk diffusion sensitivity testing. Mueller and Hinton developed this medium to be a protein free medium for isolating pathogenic strains of Neisseria1 and it was found that Mueller Hinton Agar was useful in identifying sulfonimide-resistant and responsive strains of gonococci2. Also this media has been used in standardized antimicrobial disk susceptibility testing, as described by Bauer, Kirby, et al3.

Mueller Hinton Media contains beef infusion, casamino acids, and starch. Starch acts as a colloid that protects against toxic material in the medium and agar is added when a solidifying agent is needed. The Kirby-Bauer antimicrobial disk diffusion procedure is used with Mueller Hinton Agar plates. An antimicrobial impregnated disk is placed on an agar surface, resulting in diffusion of the antimicrobial into the surrounding medium. Effectiveness of the antimicrobial can be shown by measuring the zone of inhibition for a pure culture of an organism4,5. Mueller Hinton Broth is the same formulation, without the added agar. It is used for the cultivation of microorganisms, and for making dilutions of organisms to be used in the Kirby-Bauer disk diffusion studies.

Product NameCatalog #Quantity
Mueller Hinton Agar mono plate, 90 x 15 mm CM9380P 10/pk
Mueller Hinton Slant CM9380S 10/pk
Mueller Hinton Broth CM9380B 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Escherichia coli (VRE) ATCC® 25922 Growth
Escherichia coli (VRE) ATCC® 35218 Growth
Staphylococcus aureus (VRE) ATCC® 25923 Growth
Pseudomonas aeruginosa (VRE) ATCC® 27853 Growth
Enterococcus faecalis (VRE) ATCC® 29212 Growth

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.3 +/- 0.1 at 25°C)
Acid Hydrolysate of Casein 17.5
Beef Extract 2.0
Starch 1.5
Agar (where applicable) 17.0
5 total citations

  1. Mueller, J.H. and J. Hinton. 1941. A protein-free medium for primary isolation of the Gonococcus and Meningococcus. Proc. Soc. Exp. Diol. and Med.; 48:330-333.
  2. Ryan, K.J., et al. 1970. Disk sensitivity testing. Hosp. Prac.; 5:91-100.
  3. Bauer, A.W., W.M.M. Kirby, et al. 1966. Am. J. Clin. Pathol.; 45:493-496.
  4. Performance Standards for Antimicrobial Disk Susceptibility Tests. M2-A. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.
  5. Standard Disk Susceptibility Test. The Federal Register, September 30, 1972; 37(191):20527-20529
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