Fluid Thioglycollate Medium

Fluid Thioglycollate Medium (FTM), also called Thioglycollate Medium is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganism, and is recommended for sterility checks on banked blood and blood cultures1. The presence of small amount of agar, and the presence of sodium thioglycollate which as a reducing agent maintains a low oxygen tension gives this medium its unique ability to support the growth of a large variety of fastidious microorganisms of anaerobic as well as aerobics2-4.

Certain additives can be incorporated into the Thioglycollate Medium as desired. Yeast extract or papaic digest of soybean meal may be added as growth enhancers. Hemin can be incorporated to supply X factor for the stimulated growth of many fastidious organisms, vitamin K for growth requirements some gram-positive spore-formers and Bacteroides species. Calcium carbonate chips may be added to act as a buffer for the medium and prevents buildup of toxic acids.

Product NameCatalog #Quantity
Fluid Thioglycollate Agar mono plate, 90 x 15 mm CF9340P 10/pk
Fluid Thioglycollate Slant CF9340S 10/pk
Fluid Thioglycollate Broth CF9340B 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Clostridium novyi ATCC® 7659 Growth
Staphylococcus aureus ATCC® 25923 Growth
Candida albicans ATCC® 10231 Growth
Bacillus subtilis ATCC® 6633 Growth

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.2 +/- 0.2 at 25°C)
Pancreatic Digest of Casein 15.0
Dextrose 5.0
Yeast Extract 5.0
Sodium Chloride 2.5
Sodium Thioglycollate 0.5
L-Cystine 0.25
Agar 0.75
4 total citations

  1. Isenberg, H. D. (ed.) 1992. Clinical microbiology procedures handbook, vol. 1, American Society for Microbiology, Washington, D.C.
  2. Quastel and Stephenson. 1926. General biological products standards. Fed. Regist. 21:6109.12
  3. Falk, C. R., H. Bucca, and M. P. Simmons. 1939. A comparative study of the use of varying concentrations of agar in the the medium used to detect contaminants in biological products, J. Bacteriol. 37:121-131
  4. Brewer, J. H. 1940. Clear liquid mediums for the "aerobic" cultivation of anaerobes. J. Amer. Med. Assoc. 115:598-600
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