Cooked Meat Medium

Cooked Meat Medium is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. The use of animal tissue for culturing anaerobic organisms was first employed by Theobald Smith1 later Von Hibler used brain tissue for cultivating and classifying anaerobic bacilli2. Robertson replaced brain tissue with beef heart and used this medium to differentiate putrefactive and saccharolytic species1. The formulation presently used is a modified version of Robertson's formulation and is also referred to as Chopped Meat Medium4.

Reducing substances, which permit the growth of strict anaerobes, are supplied by the muscle tissue and the iron filings5. Because reducing substances are readily available in denatured protein, the meat particles are cooked before use in the medium.

Growth of spore-forming and non-spore-forming obligate anaerobes is supported by this medium. Additionally, researchers have found that Cooked Meat Medium preserves viability of organisms over a long period of time and is useful in maintaining anaerobic stock organisms6. The Food and Drug Administration recommends its use in the enumeration and identification of Clostridium perfringens from food7.

Product NameCatalog #Quantity
Cooked Meat Agar mono plate, 90 x 15 mm CC9260P 10/pk
Cooked Meat Slant CC9260S 10/pk
Cooked Meat Broth CC9260B 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Bacteroides fragilis ATCC® 25285 Growth
Streptococcus pyogenes ATCC® 19615 Growth
Clostridium perfringens ATCC® 13124 Growth

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.2 +/- 0.2 at 25°C)
Beef Heart 454.0
Peptic Digest of Animal Tissue 10.0
Pancreatic Digest of Casein 10.0
Sodium Chloride 5.0
Dextrose 2.0
Toluidine Blue O 0.075
5 total citations

  1. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD
  2. Smith, T. 1890. Centr. Bakteriol.; 7:509
  3. Murray, P.R., et al. 1995. Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C.
  4. Holman, W.L. 1919. J. Bacteriol.; 4:149
  5. Claros, M.C., et al. 1995. J. Clin. Micro.; 33; 9:2505-2507. American Society for Microbiology
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