Cooked Meat Medium
Cooked Meat Medium is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. The use of animal tissue for culturing anaerobic organisms was first employed by Theobald Smith1 later Von Hibler used brain tissue for cultivating and classifying anaerobic bacilli2. Robertson replaced brain tissue with beef heart and used this medium to differentiate putrefactive and saccharolytic species1. The formulation presently used is a modified version of Robertson's formulation and is also referred to as Chopped Meat Medium4.
Reducing substances, which permit the growth of strict anaerobes, are supplied by the muscle tissue and the iron filings5. Because reducing substances are readily available in denatured protein, the meat particles are cooked before use in the medium.
Growth of spore-forming and non-spore-forming obligate anaerobes is supported by this medium. Additionally, researchers have found that Cooked Meat Medium preserves viability of organisms over a long period of time and is useful in maintaining anaerobic stock organisms6. The Food and Drug Administration recommends its use in the enumeration and identification of Clostridium perfringens from food7.
|Product Name||Catalog #||Quantity|
|Cooked Meat Agar mono plate, 90 x 15 mm||CC9260P||10/pk|
|Cooked Meat Slant||CC9260S||10/pk|
|Cooked Meat Broth||CC9260B||10/pk|
The following organisms are routinely used for testing for this medium.
|Bacteroides fragilis ATCC® 25285||Growth|
|Streptococcus pyogenes ATCC® 19615||Growth|
|Clostridium perfringens ATCC® 13124||Growth|
User Quality Control
Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).
Ingredients g/L(Final pH 7.2 +/- 0.2 at 25°C)
|Peptic Digest of Animal Tissue||10.0|
|Pancreatic Digest of Casein||10.0|
|Toluidine Blue O||0.075|
5 total citations
- MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD
- Smith, T. 1890. Centr. Bakteriol.; 7:509
- Murray, P.R., et al. 1995. Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C.
- Holman, W.L. 1919. J. Bacteriol.; 4:149
- Claros, M.C., et al. 1995. J. Clin. Micro.; 33; 9:2505-2507. American Society for Microbiology