Columbia Agar Base is a highly nutritive and selective medium developed by Ellner et al of Columbia University1 for the cultivation of fastidious organisms with or without the addition of blood. Columbia Agar Base media can be prepared as Blood Agar or Chocolate Agar by supplementing with 5-10% sheep, rabbit or horse blood. Columbia Blood Agar is used for isolating, cultivating and determining the hemolytic reactions of fastidious pathogenic microorganisms. The addition of antibiotics, kanamycin or gentamicin, to Columbia Agar suppresses the growth of certain Gram-negative and Gram-positive microorganisms.
|Product Name||Catalog #||Quantity|
|Columbia Agar mono plate, 90 x 15 mm||CC9250P||10/pk|
|Columbia Agar w/ 6% Horse Blood mono plate||CC9256P||10/pk|
The following organisms are routinely used for testing for this medium.
|With addition of Blood|
|Streptococcus pyogenes ATCC® 19615||Growth; beta-hemolysis|
|Streptococcus pneumonia ATCC® 6305||Growth; alpha-hemolysis|
|Staphylococcus aureus ATCC® 25923||Growth; beta-hemolysis|
|Escherichia coli ATCC® 25922||Growth|
|Peptostreptococcus anaerobius ATCC® 27337||Growth|
|Without the addition of Blood|
|Clostridium sporogenes ATCC® 19404||Growth|
|Clostridium perfringens ATCC® 13124||Growth|
User Quality Control
Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).
Ingredients g/L(Final pH 7.3 +/- 0.2 at 25°C)
|Pancreatic Digest of Casein||12.0|
|Peptic Digest of Animal Tissue||5.0|
1 total citations
- Ellner, P. D., C. J. Stoessel, E. Drakeford, and F. Vasi. 1966. A new culture medium for medical bacteriology. Am. J. Clin. Pathol. 45:502-504.