Columbia Agar

Columbia Agar Base is a highly nutritive and selective medium developed by Ellner et al of Columbia University1 for the cultivation of fastidious organisms with or without the addition of blood. Columbia Agar Base media can be prepared as Blood Agar or Chocolate Agar by supplementing with 5-10% sheep, rabbit or horse blood. Columbia Blood Agar is used for isolating, cultivating and determining the hemolytic reactions of fastidious pathogenic microorganisms. The addition of antibiotics, kanamycin or gentamicin, to Columbia Agar suppresses the growth of certain Gram-negative and Gram-positive microorganisms.

Product NameCatalog #Quantity
Columbia Agar mono plate, 90 x 15 mm CC9250P 10/pk
Columbia Agar w/ 6% Horse Blood mono plate CC9256P 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
With addition of Blood  
  Streptococcus pyogenes ATCC® 19615 Growth; beta-hemolysis
  Streptococcus pneumonia ATCC® 6305 Growth; alpha-hemolysis
  Staphylococcus aureus ATCC® 25923 Growth; beta-hemolysis
  Escherichia coli ATCC® 25922 Growth
  Peptostreptococcus anaerobius ATCC® 27337 Growth
Without the addition of Blood  
  Clostridium sporogenes ATCC® 19404 Growth
  Clostridium perfringens ATCC® 13124 Growth

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.3 +/- 0.2 at 25°C)
Pancreatic Digest of Casein 12.0
Peptic Digest of Animal Tissue 5.0
Sodium Chloride 5.0
Yeast Extract 3.0
Beef Extract 3.0
Corn Starch 1.0
Agar 13.5
1 total citations

  1. Ellner, P. D., C. J. Stoessel, E. Drakeford, and F. Vasi. 1966. A new culture medium for medical bacteriology. Am. J. Clin. Pathol. 45:502-504.
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