Brucella medium is a general purpose medium for the isolation and cultivation of Brucella species1, in particular the pathogens B. melitensis, B. abortus and B. suis, from clinical material and comestibles of animal origin2. Standard Methods for the Examination of Dairy Products recommends Brucella Agar for the cultivation of Brucella species from dairy samples1. This medium is also support luxuriant growth of fastidious microorganisms including Streptococcus pneumoniae, Streptococcus viridans, and Neisseria meningitidis. Brucella Agar also can be used as a base for the isolation of Campylobacter spp. Brucella Agar with blood is used to determine bacterial hemolytic reactions. Adding hemin, vitamin K, sheep or horse blood, provide nutrients for more fastidious organisms. Antibiotics may be added to make the media selective.
|Product Name||Catalog #||Quantity|
|Brucella Agar mono plate, 90 x 15 mm||CB9180P||10/pk|
|Brucella Agar Slant||CB9180S||10/pk|
|Brucella Agar Broth||CB9180B||10/pk|
|Brucella Agar mono plate, with hemin & vitamin K 90 x 15 mm||CB9181P||10/pk|
|Brucella Agar mono plate, 90 x 15 mm glutamine & phenol red||CB9182P||10/pk|
|Brucella Agar mono plate, 90 x 15 mm with sheep blood||CB9183P||10/pk|
The following organisms are routinely used for testing for this medium.
|Bacteroides fragilis ATCC® 25285||Growth|
|Clostridium perfringens ATCC® 13124||Growth|
|Bacteroides fragilis ATCC® 19615||Growth|
User Quality Control
Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).
Ingredients g/L(Final pH 7.0 +/- 0.2 at 25°C)
|Pancreatic Digest of Casein||10.0|
|Peptic Digest of Animal Tissue||10.0|
|Agar (where applicable):||15.0|
2 total citations
- Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.
- Murray, P.R., et al. 1995. Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C.