Brucella Medium

Brucella medium is a general purpose medium for the isolation and cultivation of Brucella species1, in particular the pathogens B. melitensis, B. abortus and B. suis, from clinical material and comestibles of animal origin2. Standard Methods for the Examination of Dairy Products recommends Brucella Agar for the cultivation of Brucella species from dairy samples1. This medium is also support luxuriant growth of fastidious microorganisms including Streptococcus pneumoniae, Streptococcus viridans, and Neisseria meningitidis. Brucella Agar also can be used as a base for the isolation of Campylobacter spp. Brucella Agar with blood is used to determine bacterial hemolytic reactions. Adding hemin, vitamin K, sheep or horse blood, provide nutrients for more fastidious organisms. Antibiotics may be added to make the media selective.

Product NameCatalog #Quantity
Brucella Agar mono plate, 90 x 15 mm CB9180P 10/pk
Brucella Agar Slant CB9180S 10/pk
Brucella Agar Broth CB9180B 10/pk
Brucella Agar mono plate, with hemin & vitamin K 90 x 15 mm CB9181P 10/pk
Brucella Agar mono plate, 90 x 15 mm glutamine & phenol red CB9182P 10/pk
Brucella Agar mono plate, 90 x 15 mm with sheep blood CB9183P 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
Bacteroides fragilis ATCC® 25285 Growth
Clostridium perfringens ATCC® 13124 Growth
Bacteroides fragilis ATCC® 19615 Growth

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 7.0 +/- 0.2 at 25°C)
Pancreatic Digest of Casein 10.0
Peptic Digest of Animal Tissue 10.0
Yeast Extract 2.0
Sodium Chloride 5.0
Dextrose 1.0
Sodium Bisulfite 0.1
Agar (where applicable): 15.0
2 total citations

  1. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.
  2. Murray, P.R., et al. 1995. Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C.
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