Bordet-Gengou Agar

Bordet Gengou Agar, with the addition of glycerol and sterile blood, is used for the detection and isolation of Bordetella pertussis and Bordetella parapertussis from clinical specimens. Bordetella pertussis is the responsible for whooping cough1,2. The most specific method of detection of B. pertussis is considered to be culturing of the specimens during the clinical testing2. This medium was developed in early 1900s as an improved method for maintaining stock of Bordetella spp by Bordet and Gengou3. Kendrick and Eldering replaced the 50% human or rabbit blood in the original formulation with 15% sheep blood for routine clinical procedures4. Defibrinated sheep blood enables the detection of hemolytic reactions, which aid in the identification of B. pertussis.

Product NameCatalog #Quantity
Bordet Gengou Agar mono plate, 90 x 15 mm CB9160P 10/pk
Bordet Gengou Agar Slant CB9160S 10/pk
Bordet Gengou Broth CB9160B 10/pk

Quality Control

The following organisms are routinely used for testing for this medium.
Test OrganismsResults
aBordetella pertussis ATCC® 9797 Growth
aBordetella parapertussis ATCC® 15311 Growth
aRecommended QC strains for User Quality Control according to the CLSI document M22 when applicable.

User Quality Control

Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).

Ingredients g/L

(Final pH 6.7 +/- 0.2 at 25°C)Pancreatic Digest of Casein5.0
Sodium Chloride 5.5
Peptic Digest of Animal Tissue 5.0
Potato Infusion 4.5
Sheep Blood (mL) 150.0
Glycerol 10.0
Agar (where applicable): 15.0
4 total citations

  1. Forbes, B.A., et al. 2007. Bailey and Scott's Diagnostic Microbiology, 12th ed. C.V. Mosby Company, St. Louis, MO.
  2. Murray, P.R., et al. 2007. Manual of Clinical Microbiology, 9th ed. American Society for Microbiology, Washington, D.C.
  3. Bordet, J. and D. Gengou. 1906. Le microbe de la coqueluche. Ann. Inst. Pasteur. 20:731.
  4. Kendrick, P. and G. Eldering. 1933. Cough Plate Examinations for B. pertussis. APHL 62nd Annual Meeting. Indianapolis, IN.
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