Bordet Gengou Agar, with the addition of glycerol and sterile blood, is used for the detection and isolation of Bordetella pertussis and Bordetella parapertussis from clinical specimens. Bordetella pertussis is the responsible for whooping cough1,2. The most specific method of detection of B. pertussis is considered to be culturing of the specimens during the clinical testing2. This medium was developed in early 1900s as an improved method for maintaining stock of Bordetella spp by Bordet and Gengou3. Kendrick and Eldering replaced the 50% human or rabbit blood in the original formulation with 15% sheep blood for routine clinical procedures4. Defibrinated sheep blood enables the detection of hemolytic reactions, which aid in the identification of B. pertussis.
|Product Name||Catalog #||Quantity|
|Bordet Gengou Agar mono plate, 90 x 15 mm||CB9160P||10/pk|
|Bordet Gengou Agar Slant||CB9160S||10/pk|
|Bordet Gengou Broth||CB9160B||10/pk|
The following organisms are routinely used for testing for this medium.
|aBordetella pertussis ATCC® 9797||Growth|
|aBordetella parapertussis ATCC® 15311||Growth|
User Quality Control
Check for signs of contamination and deterioration. Users of commercially prepared media may be required to perform quality control testing with at least one known organism to demonstrate growth or a positive reaction; and at least one organism to demonstrate inhibition or a negative reaction (where applicable).
Ingredients g/L(Final pH 6.7 +/- 0.2 at 25°C)Pancreatic Digest of Casein5.0
|Peptic Digest of Animal Tissue||5.0|
|Sheep Blood (mL)||150.0|
|Agar (where applicable):||15.0|
4 total citations
- Forbes, B.A., et al. 2007. Bailey and Scott's Diagnostic Microbiology, 12th ed. C.V. Mosby Company, St. Louis, MO.
- Murray, P.R., et al. 2007. Manual of Clinical Microbiology, 9th ed. American Society for Microbiology, Washington, D.C.
- Bordet, J. and D. Gengou. 1906. Le microbe de la coqueluche. Ann. Inst. Pasteur. 20:731.
- Kendrick, P. and G. Eldering. 1933. Cough Plate Examinations for B. pertussis. APHL 62nd Annual Meeting. Indianapolis, IN.