Antibiotics & Antimycotics

An extensive line of antibiotics and antimycotics commonly used in cell culture applications are currently offered by UFC Biotechnology for the prophylactic control or the elimination of a broad spectrum of bacterial and mycotic contaminants from mammalian and insect cell cultures. Care should be exercised in choosing the appropriate concentration of an antibiotic, since many antibiotics may exert cytotoxic effects in specific cell culture systems at concentrations minimally higher than the recommended dosage. Some of the antibiotics act synergistically, and combinations may be toxic at concentrations below those recommended for individual use. UFC Biotechnology offers high quality antibiotics with purity levels exceeding 95% and with the best prices on the market. Our antibiotics are available in solution filtered to sterility for customer convenience and validated for cell culture usage. The antibiotics offered are excellent choices of antibiotics for selection in both mammalian cells and E. coli. G418 is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. As an analog of neomycin sulfate, it interferes with the function of 80S ribosomes and protein synthesis in eukaryotic cells. G418 is commonly used as a selective agent for eukaryotic cells. Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘II. The dominant-acting resistance genes (neor) used on common mammalian expression vectors contain elements derived from either transposons Tn601 (903) or Tn5. When grown in medium containing G418 selection agent, stable colonies of mammalian cells expressing these resistance markers can be generated in 10 to 14 days. Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants. Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. Adherent mammalian cells are sensitive to concentrations of 2 to 5 µg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 µg/ml. Puromycin-resistant stable mammalian cell lines can be generated in less than one week.

Product Storage Form Catalog #
Amphotericin B solution, 250 μg/mL   Liquid A139789-250 -10ML
Ampicillin sodium salt, USP grade   Powder A695230-USP -10G
G418 sulfate -20C Powder G108321-000 -10G
Gentamicin sulfate, USP grade -20C Powder G140541-USP -5G
Kanamycin monosulfate USP grade -20C Powder K258399-USP -5G
Puromycin dihydrochloride   Powder P585820-000 -1G
Amphotericin B solution, 250 μg/mL -20C Liquid A139789-250 -10ML
Penicillin-Streptomycin solution, 100X -20C Powder T-PNST-100X -100ML
Antibiotic-Antimycotic (penicillin, streptomycin, amphotericin B) solution, 100X -20C Liquid T-ANAN-100X -100ML
G418 solution, 1g/mL -20C Liquid P585820-000 -1G
Gentamicin solution, 10 mg/mL -20C Liquid G140541-010 -10ML
Gentamicin solution, 50 mg/mL -20C Liquid G140541-050 -10ML
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