Medium 199

Medium 199 is the first fully defined, animal origin-free medium. This is an often used medium for the culture of various non-transformed cells, vaccine production, and virology. Long-term use of this medium requires serum supplementation. When supplemented with serum, this medium can be used for wide variety of cells. Media 199 are formulated with either Hank’s salts or Earle’s salts.


With Earl’s Salts
Catalog # Quantity Form NaHCO3 Glutamine Phenol Red HEPES
T-199E-1110 -500ML Liquid ✓L-Glu
T-199E-1110 -500ML Liquid
T-199E-0010 -500ML Liquid
T-199E-0110 -500ML Liquid ✓L-Glu
T-199E-0000 -500ML Liquid
T-199E-1011 -500ML Liquid ✓25mM
T-199E-0111 -500ML Liquid ✓L-Glu ✓25mM


With Hank’s Salts
Catalog # Quantity Form NaHCO3 Glutamine Phenol Red
T-199H-101 -500ML Liquid  
T-199H-001 -500ML Liquid
T-199H-011 -500ML Liquid ✓ L-Glu
T-199H-000 -500ML Liquid


McCoys 5A
Catalog # Quantity Form NaHCO3 Glutamine Phenol Red HEPES
T-MCY5-1110 -500ML Liquid ✓L-Glu
T-MCY5-1111 -500ML Liquid ✓L-Glu ✓25mM
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